Abstract
The aim of this study was to determine the pancreatic amylase activity of 24 female Wistar rats fed with cassava- cyanide diet and as well as the in-vivo effect of Malus domestica and Moringa-oleifera seed. Photochemical constituent of Moringa-oleifera have proven to have hypotenstive, anticancer and antibacterial activity and in Malus domestica it is reported to have very strong antioxidant and lower cholesterol. The result indicates the assay for pancreatic amylase which were determined to be 109.5± 2. 08 for control group 1a following 110. 76±2.06 µL-1 control 1b others were 122.50 ± 3.0 µL-1, 11 7.75± 2.06 µL-1 and 120.0±2.16 µL-1 for groups 2 to 5 respectively. Group 3 and 5 had the highest enzyme activity mean which show that pancreatic amylase was affected when test groups were fed with diets containing cassava- cyanide but Malus domestica and Moringa-oleifera seed reduced the cyanide in the pancreases in the decreasing order Moringa-oleifera seed is greater than Malus domestica.Pancreatic amylase assay was done as describe by Agape Kit.
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